Volume 7, Issue 2 (December 2021)                   Elderly Health Journal 2021, 7(2): 79-83 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Soleimani H, Rezvani M E, Hafizi-Barjin Z, Esmaeilidehaj M, Zaremehrjerdi F. Effects of Chlorogenic Acid on Epileptic Behavior and mRNA Expressions of Brain Derived Neurotrophic Factor in the Brain of Aged Rats. Elderly Health Journal. 2021; 7 (2) :79-83
URL: http://ehj.ssu.ac.ir/article-1-125-en.html
Department of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , erezvani@yahoo.com
Abstract:   (233 Views)
Introduction: The present study was conducted to evaluate the effect of Chlorogenic Acid (CA) and Diazepam (DZP) on epileptic complication that induced by repetitive intra-peritoneal injections of Pentylenetetrazle (PTZ) in aged rats.
 
Methods: Twenty-four month-old male Wistar rats (age > 12 months, 300-350 g) were divided in 4 experimental groups. Animal in control group (PTZ + Vehicle) received only PTZ. Animal in treated groups (PTZ + DZP, PTZ + CA10 and PTZ + 25) received diazepam 2 mg/kg, CA 10 mg/kg, or CA 25 mg/kg. All drugs injection were performed  30 min prior to each PTZ injection. Epilepsy was induced by injection of subconvulsive dose of PTZ every other day until the rats were completely kindled or epileptic. After each PTZ injection, animal was monitored for 40 min and epileptic behaviors were scored. At the end of the study, rats were sacrificed and the brains removed for evaluation of histological changes and Brain Derived Neurothrophic Factor (BDNF) gene expression.
 
Results: CA at dose of 25 mg/kg reduced percent of Generalized Tonic-Clonic Seizure (GTCS) in aged rats (24%) in compared to control group (50%) (p < 0.05). The latencies to the start of GTCS were decreased in both dose of CA (p < 0.05). Also, the percent of survived neurons in rats treated with CA (154%) were significantly higher relative to that of control animals (100%) (p < 0.05). The mRNA levels of BDNF significantly increased in CA treated rats (p < 0.05).
 
Conclusion: Hence, these findings revealed that CA have antiepileptic, neuroprotective and trophic effects in aged rats. CA can protect aged brain from deteriorative processes and save neurons during epilepsy in rats.
Full-Text [PDF 1297 kb]   (89 Downloads) |   |   Full-Text (HTML)  (72 Views)  
Type of Study: Research | Subject: General
Received: 2018/10/7 | Accepted: 2021/10/19 | Published: 2021/12/19

References
1. Hernández-Ronquillo L, Adams S, Ballendine S, Téllez-Zenteno JF. Epilepsy in an elderly population: Classification, etiology and drug resistance. Epilepsy Research. 2018; 140: 90-4.
2. Erdő F, Denes L, de Lange E. Age-associated physiological and pathological changes at the blood–brain barrier: a review. Journal of Cerebral Blood Flow & Metabolism. 2017; 37(1): 4-24.
3. Mercado NM, Collier TJ, Sortwell CE, Steece-Collier K. BDNF in the aged brain: translational implications for Parkinson’s disease. Austin Neurology & Neurosciences. 2017; 2(2).
4. Mariga A, Mitre M, Chao MV. Consequences of brain-derived neurotrophic factor withdrawal in CNS neurons and implications in disease. Neurobiology of Disease. 2017; 97: 73-9.
5. Brunton L, Chabner B, Knollman B. Goodman and Gilman's the pharmacological basis of therapeutics. New York: McGraw-Hill; 1996.
6. Bethmann K, Fritschy JM, Brandt C, Löscher W. Antiepileptic drug resistant rats differ from drug responsive rats in GABAA receptor subunit expression in a model of temporal lobe epilepsy. Neurobiology of Disease. 2008; 31(2): 169-87.
7. Ali I, Chugh D, Ekdahl CT. Role of fractalkine–CX3CR1 pathway in seizure-induced microglial activation, neurodegeneration, and neuroblast production in the adult rat brain. Neurobiology of Disease. 2015; 74:194-203.
8. Bravo L. Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutrition Reviews. 1998; 56(11): 317-33.
9. Basli A, Soulet S, Chaher N, Mérillon JM, Chibane M, Monti JP, et al. Wine polyphenols: potential agents in neuroprotection. Oxidative Medicine and Cellular Longevity. 2012; 2012: 1-14.
10. Ozcan T, Akpinar-Bayizit A, Yilmaz-Ersan L, Delikanli B. Phenolics in human health. International Journal of Chemical Engineering and Applications. 2014; 5(5): 393-6.
11. Aseervatham SB, Suryakala U, Nisha D, Sundaram S, Bose PC, Sivasudha T. Expression pattern of NMDA receptors reveals antiepileptic potential of apigenin 8-C-glucoside and chlorogenic acid in pilocarpine induced epileptic mice. Biomedicine & Pharmacotherapy. 2016; 82: 54-64.
12. Szwajgier D, Borowiec K, Pustelniak K. The neuroprotective effects of phenolic acids: Molecular mechanism of action. Nutrients. 2017; 9(5): 477.
13. Cevik B, Solmaz V, Aksoy D, Erbas O. Montelukast inhibits pentylenetetrazol-induced seizures in rats. Medical Science Monitor. 2015; 21: 869-74.
14. Huang JL, Jing X, Tian X, Qin MC, Xu ZH, Wu DP, et al. Neuroprotective properties of Panax notoginseng saponins via preventing oxidative stress injury in SAMP8 mice. Evidence-Based Complementary and Alternative Medicine. 2017; 2017: 1-8.
15. Wilson JX, Gelb AW. Free radicals, antioxidants, and neurologic injury: possible relationship to cerebral protection by anesthetics. Journal of Neurosurgical Anesthesiology. 2002; 14(1): 66-79.
16. Ataie A, Ataee R, Shadifar M. Polyphenolic antioxidants and neuronal regeneration. Basic and Clinical Neuroscience. 2016; 7(2): 81-90.
17. Agunloye OM, Oboh G. Caffeic acid and chlorogenic acid: Evaluation of antioxidant effect and inhibition of key enzymes linked with hypertension. Journal of Food Biochemistry. 2018; 42(4): e12541.
18. Akila P, Vennila L. Chlorogenic acid a dietary polyphenol attenuates isoproterenol induced myocardial oxidative stress in rat myocardium: An in vivo study. Biomedicine & Pharmacotherapy. 2016; 84: 208-14.
19. Meng S, Cao J, Feng Q, Peng J, Hu Y. Roles of chlorogenic acid on regulating glucose and lipids metabolism: a review. Evidence-Based Complementary and Alternative Medicine. 2013; 2013.
20. Naveed M, Hejazi V, Abbas M, Kamboh AA, Khan GJ, Shumzaid M, et al. Chlorogenic acid (CGA): A pharmacological review and call for further research. Biomedicine & Pharmacotherapy. 2018; 97:67-74.
21. Ambrogini P, Torquato P, Bartolini D, Albertini MC, Lattanzi D, Di Palma M, et al. Excitotoxicity, neuroinflammation and oxidant stress as molecular bases of epileptogenesis and epilepsy-derived neurodegeneration: The role of vitamin E. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 2019; 1865(6): 1098-112.
22. Paredes RG, Ågmo A. GABA and behavior: the role of receptor subtypes. Neuroscience & Biobehavioral Reviews. 1992; 16(2): 145-70.
23. 23. Binder DK. The role of BDNF in epilepsy and other diseases of the mature nervous system. In: Binder DK, Scharfman HE, editors. Recent advances in epilepsy research. Advances in experimental medicine and biology. vol 548. Boston: Springer; 2004. p. 34-56.
24. Palma E, Torchia G, Limatola C, Trettel F, Arcella A, Cantore G, et al. BDNF modulates GABAA receptors microtransplanted from the human epileptic brain to Xenopus oocytes. Proceedings of the National Academy of Sciences. 2005; 102(5): 1667-72.

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Elderly Health Journal

Designed & Developed by : Yektaweb