Volume 7, Issue 2 (December 2021)
Elderly Health Journal 2021, 7(2): 71-78 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
jafari M, Hosseinpour Delavar S, Safikhani H, Azizi M. The Effect of Eight Weeks of Continuous and Interval Training with Citrus Aurantium Consumption on Autophagy Markers and MyoD Activation in the Muscle Tissue of Elderly Rats. Elderly Health Journal 2021; 7 (2) :71-78
URL: http://ehj.ssu.ac.ir/article-1-226-en.html
URL: http://ehj.ssu.ac.ir/article-1-226-en.html
Department of Exercise Physiology, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran , delavar2009@iauksh.ac.ir
Abstract: (1230 Views)
Introduction: Although exercise training and herbs consumption have protective effects on many diseases, the mechanism of action of exercise training with different intensities and citrus aurantium (CA) extract consumption on the autophagy-dependent MyoD activation pathway is not yet known. The aim of this study was to investigate the effect of eight weeks of moderate intensity continuous training (MICT) and high intensity interval training (HIIT) with CA consumption on the expression of LC3-II, Beclin-1 and MyoD as autophagy related markers in the muscle tissue of elderly rats.
Methods: In this experimental study, 42 elderly female rats were randomly assigned to (1) control (C) (2) MICT, (3) HIIT, (4) MICT + CA, (5) HIIT + CA, (6) CA and (7) sham (normal saline) groups. HIIT was performed at 85-110% VO2max intensity and 15-25 m / min speed and MICT at 65% VO2max intensity and 20-25 m / min speed; 300 mg / kg / day CA was received peritoneally. One-way analysis of variance with Tukey's post hoc test was used to analyze the findings. Findings were analyzed using Graph Pad Prism 8.3.0 software (p ≤ 0.05).
Results: MICT and HIIT increased LC3II, Bclin1 and MyoD gene expression (p ≤ 0.05); The effect of HIIT on MyoD increase was greater than MICT (p ≤ 0.05). CA increased the expression of LC3II and Bclin1 (p ≤ 0.05). MICT + CA and HIIT + CA increased the expression of LC3II, Bclin1 and MyoD in the muscle tissue of elderly rats (p ≤ 0.05).
Conclusion: It seems that exercise training and CA consumption with different mechanism of action activate autophagy in the soleus muscle tissue, however the simultaneous use of HIIT, MICT and CA also has favorable effects on the autophagy-dependent MyoD activation pathway.
Methods: In this experimental study, 42 elderly female rats were randomly assigned to (1) control (C) (2) MICT, (3) HIIT, (4) MICT + CA, (5) HIIT + CA, (6) CA and (7) sham (normal saline) groups. HIIT was performed at 85-110% VO2max intensity and 15-25 m / min speed and MICT at 65% VO2max intensity and 20-25 m / min speed; 300 mg / kg / day CA was received peritoneally. One-way analysis of variance with Tukey's post hoc test was used to analyze the findings. Findings were analyzed using Graph Pad Prism 8.3.0 software (p ≤ 0.05).
Results: MICT and HIIT increased LC3II, Bclin1 and MyoD gene expression (p ≤ 0.05); The effect of HIIT on MyoD increase was greater than MICT (p ≤ 0.05). CA increased the expression of LC3II and Bclin1 (p ≤ 0.05). MICT + CA and HIIT + CA increased the expression of LC3II, Bclin1 and MyoD in the muscle tissue of elderly rats (p ≤ 0.05).
Conclusion: It seems that exercise training and CA consumption with different mechanism of action activate autophagy in the soleus muscle tissue, however the simultaneous use of HIIT, MICT and CA also has favorable effects on the autophagy-dependent MyoD activation pathway.
Type of Study: Research |
Subject:
General
Received: 2021/04/3 | Accepted: 2021/09/14 | Published: 2021/12/19
Received: 2021/04/3 | Accepted: 2021/09/14 | Published: 2021/12/19
References
1. Segalés J, Perdiguero E, Serrano AL, Sousa-Victor P, Ortet L, Jardí M, et al. Sestrin prevents atrophy of disused and aging muscles by integrating anabolic and catabolic signals. Nature Communications. 2020; 11(1): 1–13.
2. Wilkinson DJ, Piasecki M, Atherton PJ. The age-related loss of skeletal muscle mass and function: Measurement and physiology of muscle fibre atrophy and muscle fibre loss in humans. Ageing Research Reviews. 2018; 47: 123–32.
3. Rong S, Wang L, Peng Z, Liao Y, Li D, Yang X, et al. The mechanisms and treatments for sarcopenia: could exosomes be a perspective research strategy in the future?. Journal of Cachexia Sarcopenia Muscle. 2020; 11(2): 1-18.
4. Liang J, Zeng Z, Zhang Y, Chen N. Regulatory role of exercise-induced autophagy for sarcopenia. Experimental Gerontology. 2020; 130: 110789.
5. Park SS, Seo Y-K, Kwon K-S. Sarcopenia targeting with autophagy mechanism by exercise. BMB Reports. 2019; 52(1):64.
6. Liu C-W, Huang C-C, Hsu C-F, Li T-H, Tsai Y-L, Lin M-W, et al. SIRT1-dependent mechanisms and effects of resveratrol for amelioration of muscle wasting in NASH mice. BMJ Open Gastroenterology. 2020; 7(1): 1-12.
7. Han S, Cui C, He H, Shen X, Chen Y, Wang Y, et al. FHL1 regulates myoblast differentiation and autophagy through its interaction with LC3. Journal of Cellular Physiology. 2020; 235(5): 4667–78.
8. Kim YA, Kim YS, Oh SL, Kim H-J, Song W. Autophagic response to exercise training in skeletal muscle with age. Journal of Physiology and Biochemistry. 2013; 69(4): 697–705.
9. Weng T-P, Huang S-C, Chuang Y-F, Wang J-S. Effects of interval and continuous exercise training on CD4 lymphocyte apoptotic and autophagic responses to hypoxic stress in sedentary men. PLoS One. 2013; 8(11): 1-17.
10. Mejías-Peña Y, Estébanez B, Rodriguez-Miguelez P, Fernandez-Gonzalo R, Almar M, de Paz JA, et al. Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects. Aging. 2017: 9(2): 408–18.
11. Narasimhan M, Hong J, Atieno N, Muthusamy VR, Davidson CJ, Abu-Rmaileh N, et al. Nrf2 deficiency promotes apoptosis and impairs PAX7/MyoD expression in aging skeletal muscle cells. Free Radical Biology and Medicine. 2014; 71: 402–14.
12. Alfarafisa NM, Kitaguchi K, Yabe T. The aging of skeletal muscle and potential therapeutic effects of extracts from edible and inedible plants. Reviews in Agricultural Science. 2020; 8: 70–88.
13. Chaabane M, Elwej A, Ghorbel I, Boudawara T, Zeghal N, Soudani N. Citrus aurantium L. peel extract mitigates hexavalent chromium-induced oxidative stress and cardiotoxicity in adult rats. Pharmaceutical and Biomedical Research. 2017; 3(2): 8-18.
14. He W, Li Y, Liu M, Yu H, Chen Q, Chen Y, et al. Citrus aurantium L. and its flavonoids regulate TNBS-induced inflammatory bowel disease through anti-inflammation and suppressing isolated jejunum contraction. International Journal of Molecular Sciences. 2018; 19(10): 1-14.
15. Hosseini SA, Salehi O, Keikhosravi F, Hassanpour G, Ardakani HD, Farkhaie F, et al. Mental health benefits of exercise and genistein in elderly rats. Experimental Aging Research. 2021; 1–16.
16. Sengupta P. The laboratory rat: relating its age with human’s. International Journal of Preventive Medicine. 2013; 4(6): 624-30.
17. Yazdanparast Chaharmahali B, Azarbayjani MA, Peeri M, Farzanegi Arkhazloo P. The Effect of moderate and high intensity interval trainings on cardiac apoptosis in the old female rats. Report of Health Care Journal. 2018; 4(1): 26–35.
18. Li F-H, Sun L, Zhu M, Li T, Gao H-E, Wu D-S, et al. Beneficial alterations in body composition, physical performance, oxidative stress, inflammatory markers, and adipocytokines induced by long-term high-intensity interval training in an aged rat model. Experimental Gerontology. 2018; 113: 150–62.
19. Lee DE, Bareja A, Bartlett DB, White JP. Autophagy as a therapeutic target to enhance aged muscle regeneration. Cells. 2019; 8(2): 183.
20. Luo L, Lu A-M, Wang Y, Hong A, Chen Y, Hu J, et al. Chronic resistance training activates autophagy and reduces apoptosis of muscle cells by modulating IGF-1 and its receptors, Akt/mTOR and Akt/FOXO3a signaling in aged rats. Experimental Gerontology. 2013; 48(4): 427–36.
21. Li F-H, Sun L, Wu D-S, Gao H-E, Min Z. Proteomics-based identification of different training adaptations of aged skeletal muscle following long-term high-intensity interval and moderate-intensity continuous training in aged rats. Aging. 2019; 11(12): 4159–82.
22. Ramezannezhad P, Heidari-Soureshjani S, Suhan T. Protective effects of some medicinal plants against myocardial hypoxia. International Journal of Biology and Chemistry. 2019; 12(1): 112–27.
23. Lee HJ, Venkatarame Gowda Saralamma V, Kim SM, Ha SE, Raha S, Lee WS, et al. Pectolinarigenin induced cell cycle arrest, autophagy, and apoptosis in gastric cancer cell via PI3K/AKT/mTOR signaling pathway. Nutrients. 2018; 10(8): 1-15.
24. Raha S, Yumnam S, Hong GE, Lee HJ, Saralamma VVG, Park H-S, et al. Naringin induces autophagy-mediated growth inhibition by downregulating the PI3K/Akt/mTOR cascade via activation of MAPK pathways in AGS cancer cells. International Journal of Oncology. 2015; 47(3): 1061–9.
25. Hansen DK, George NI, White GE, Abdel-Rahman A, Pellicore LS, Fabricant D. Cardiovascular toxicity of Citrus aurantium in exercised rats. Cardiovascular Toxicology. 2013; 13(3): 208–19.
26. Colker CM, Kaiman DS, Torina GC, Perlis T, Street C. Effects of Citrus aurantium extract, caffeine, and St. John’s wort on body fat loss, lipid levels, and mood states in overweight healthy adults. Current Therapeutic Research. 1999; 60(3): 145–53.
27. Kliszczewicz B, Bechke E, Williamson C, Bailey P, Hoffstetter W, McLester J, et al. The influence of citrus aurantium and caffeine complex versus placebo on the cardiac autonomic response: a double blind crossover design. Journal of the International Society of Sports Nutrition. 2018; 15(34): 1–8.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
